What is Phosphate Buffered Saline (PBS) and what are its components?

Phosphate Buffered Saline (PBS) is a buffer solution commonly used in biological research and clinical applications. The key components include sodium phosphate, sodium chloride, and water, and it is specifically formulated to maintain a stable pH and osmotic balance. The absence of calcium and magnesium ions within this formulation makes it particularly useful in various applications, such as cell culture and protein washings, where the presence of these ions could interfere with experimental results.

Why should I choose PBS without calcium and magnesium?

Choosing a phosphate buffered saline without calcium and magnesium is crucial for applications that require ionic-free environments. Calcium and magnesium can affect cellular processes and interactions, leading to inconsistent results in experiments, particularly in cell detachment or during enzyme reactions. Skipping these ions ensures that cellular pathways remain unaffected during experimentation, making it ideal for sensitive biological assays or research into protein-protein interactions.

How should I store 21-040-CV Phosphate Buffered Saline?

To maintain the integrity of 21-040-CV Phosphate Buffered Saline, it should be stored at a temperature between 2°C to 8°C, preferably in a dark place away from direct sunlight. This prevents the degradation of the buffer components and maintains its effectiveness over time. Always check the solution for any visible changes prior to use to ensure that it remains clear and free of contamination.

Can I use PBS for cell culture?

Yes, PBS is widely used for cell culture applications, particularly for washing cells and diluting substances in various biological assays. The balanced ionic environment it provides helps to maintain cell integrity during procedures such as passaging or trypsinization. Its use in cell culture ensures that cells remain viable and can effectively replicate without interference from ions that could potentially alter their behavior.

What are the best uses for the 500 mL pack of PBS?

The 500 mL pack of PBS is ideal for laboratories or research facilities that require a moderately sized volume for multiple assays or experiments. Its versatility allows researchers to use it for washing cells, diluting samples, or serving as a buffer in various biochemical procedures. This volume is suitable for routine laboratory work, making it convenient for regular experimentation without the need for constant replenishment.

Is the PBS solution sterile?

The 21-040-CV Phosphate Buffered Saline is typically supplied as a sterile solution, intended for use in laboratory settings where contamination must be minimized. Sterile PBS is crucial for maintaining cell viability and integrity during cell culture, as well as for ensuring that experimental results are reliable and accurate. Always consult the label or product specification to confirm the sterility of the batch before use.

What are some practical applications of PBS in research?

Phosphate Buffered Saline is used in a myriad of applications in research, including as a washing solution for cells, diluent for antibodies and proteins, and with immunoassays. Its buffering capability helps to maintain the pH during various protocols, making it essential in experiments such as flow cytometry, enzyme assays, and immunohistochemistry. Researchers appreciate its ability to create a controlled environment, ensuring that biological samples remain unaffected during procedures.

How long can I store an open vial of PBS?

An open vial of phosphate buffered saline should be stored in refrigerated conditions and utilized within a reasonable timeframe to maintain its integrity, typically within a few weeks. Beyond this period, PBS may begin to lose its effectiveness. Always inspect the solution for turbidity or any changes in color, which can indicate contamination. Proper labeling and tracking of the produced date can help maintain its usability.

Can 21-040-CV PBS be used for in vivo studies?

While Phosphate Buffered Saline is primarily used for in vitro studies, it can be applied in some in vivo studies as a vehicle for the delivery of drugs or other agents. However, it’s important to consider the specific requirements of the study, as PBS may not be suitable for all in vivo applications due to its composition. Careful evaluation of the interplay between PBS and biological systems is recommended before administration.

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Item #: 11980420

Mediatech 21-040-CV Phosphate Buffered Saline, 1X, Without Calcium and Magnesium, 500 mL, 500 mm (Pack of 6)

Q: Where can I buy 21-040-CV Phosphate Buffered Saline, 1X, Without Calcium and Magnesium, 500 mL, Pack of 6?

You can purchase the 21-040-CV Phosphate Buffered Saline, 1X, Without Calcium and Magnesium, 500 mL, Pack of 6 on Ubuy. Ubuy offers a convenient online shopping experience and provides a wide selection of lab supplies, ensuring that you have access to quality products for your research needs. Please visit their website to check availability and make your purchase.

Item #: 11980420

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What Stands Out

Buffer Stability

This phosphate buffered saline maintains a precise pH, ensuring stability in biological and chemical experiments, making it ideal for laboratory use.

Calcium-Free Formula

Without calcium and magnesium, this saline solution minimizes cellular interactions, providing a pure environment for sensitive cell culture applications.

Bulk Pack Size

Packaged in six 500 mL bottles, this product offers convenience and cost-effectiveness, reducing the need for frequent reordering and ensuring ample supply for extended experiments.

Product Details

Mediatech 21-040-CV Phosphate Buffered Saline, 1X, without Calcium and Magnesium
500 mL, 500 mm Pack of 6
Lab supply for maintaining cell culture media
Buffer solution for maintaining pH range of 7.0 to 7.6
Can be used as an irrigating, transporting, or diluting fluid
Ensures cell tonicity and viability for a limited period of time

Package Weight

9.3 Pound

Who Should Buy?

Cell Culture
Ideal for supporting cell viability and maintenance in various cell culture applications without the interference of calcium or magnesium.
Protein Research
Suitable for diluting proteins and antibodies for assays, providing a stable and consistent environment for reactions.
Laboratory Use
Useful in laboratories requiring a reliable phosphate buffer for biochemical experiments, enhancing reproducibility and accuracy.

Calcium-Dependent Studies
Not suitable for experiments that require calcium and magnesium, potentially leading to unreliable results in these contexts.

Product Description

Mediatech 21-040-CV Phosphate Buffered Saline, 1X, Without Calcium and Magnesium, 500 mL, 500 mm (Pack of 6)

About This Item

Looking for a reliable and essential solution to maintain your cell culture media? Look no further than Mediatech 21-040-CV Phosphate Buffered Saline! Our Mediatech Phosphate Buffered Saline, also known as Dulbecco's Phosphate Buffered Saline (DPBS), is a highly effective buffer solution that helps you maintain the physiological pH range of 7.0 to 7.6 in your cell culture media. Whether you need it as an irrigating, transporting, or diluting fluid, our product ensures your cell tonicity and viability for a limited period of time. It is the perfect solution to keep your cells healthy and thriving.

What sets our Mediatech Phosphate Buffered Saline apart is that it comes in a convenient pack of 6, each containing 500 mL of solution. With this pack, you can rest assured that you have an ample supply to meet your needs. In addition, our Phosphate Buffered Saline is specifically formulated without calcium and magnesium, making it suitable for certain cell culture applications.

Trust Mediatech, a leading provider of cell culture media solutions, to deliver high-quality and reliable products. Order your pack of Mediatech 21-040-CV Phosphate Buffered Saline today and ensure the optimal health of your cell cultures!.

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Customer Questions & Answers

Question: How to Shop Mediatech 21-040-CV Phosphate Buffered Saline, 1X, Online From Ubuy?

Answer: It’s easy to shop Mediatech 21-040-CV Phosphate Buffered Saline, 1X, online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Mediatech 21-040-CV Phosphate Buffered Saline, 1X, Available to Shop Online in Montenegro?

Answer: Yes, at Ubuy Montenegro this product is available for you to shop at a reasonable price. The Mediatech 21-040-CV Phosphate Buffered Saline, 1X, is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

High quality buffering solution
Ideal for laboratory use
Convenient pack of six
Free from calcium and magnesium
User-friendly and versatile

Cons

Packaging could be more eco-friendly.

Product Price History

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Mediatech 21-040-CV Phosphate Buffered Saline, 1X, Without Calcium and Magnesium, 500 mL, 500 mm (Pack of 6)

€ 90

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Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Product Details

Who Should Buy?

Product Description

About This Item

Customer Questions & Answers

Mediatech Buffers Editorial Review

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Pros

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Product Price History

Important information

€ 90

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