What is Dulbecco's Phosphate Buffered Saline (DPBS)?

Dulbecco's Phosphate Buffered Saline (DPBS) is a balanced salt solution commonly used in laboratory settings to maintain physiological pH and osmotic balance. This specific formulation is designed without calcium and magnesium, making it ideal for cell culture work where those ions could interfere with certain cell lines. Researchers often use DPBS for washing cells, diluting samples, or reconstituting cells in culture, ensuring that the environment remains stable for optimal cell growth.

Why is calcium and magnesium omitted from this DPBS formulation?

Calcium and magnesium are omitted from this formulation to prevent potential cell aggregation or clumping, which can occur during certain biological procedures. This makes Dulbecco's Phosphate Buffered Saline Without Calcium and Magnesium particularly suitable for cell types that require specific handling conditions or for experiments that necessitate strict control of the ionic environment. It effectively ensures that cells can be manipulated without the risk of unwanted interactions.

What is the pH level of this solution and why is it important?

The pH level of this Dulbecco's Phosphate Buffered Saline is precisely maintained at 7.4. This neutral pH is critical as it closely mimics the physiological pH of human blood, offering an optimal environment for cell culture and biological assays. Maintaining a consistent pH helps prevent cellular stress or changes in cellular behavior, which can significantly alter the outcomes of experimental results.

In what applications is DPBS typically used?

DPBS is widely utilized in various applications including cell culture, tissue storage, and as a diluent for other reagents. It serves as an excellent wash solution for cells before transferring them into fresh media, helping to remove any residual serum or substances that could affect experimental outcomes. Researchers also use it for diluting solutions during immunoassays or other biochemical analyses, ensuring optimal conditions for their specific applications.

How should Dulbecco's Phosphate Buffered Saline be stored?

Dulbecco's Phosphate Buffered Saline should be stored at 2-8 degrees Celsius, away from light to preserve its quality. For long-term storage, the solution can be kept in refrigerated conditions, but it is essential to avoid freezing, which could compromise its integrity. Proper storage prevents microbial contamination and maintains the stability of the buffer, thus ensuring consistent performance in laboratory applications.

Is Dulbecco's Phosphate Buffered Saline suitable for all cell types?

While DPBS is versatile and used for various cell types, it is crucial to verify that the specific cell line being used does not have special requirements for ions like calcium or magnesium. Certain cell types may depend on these ions for attachment or growth, so it's essential to consult specific protocols for those cell lines. However, for many standard applications, DPBS is an excellent choice for maintaining cell viability and functionality.

Can you use Dulbecco's Phosphate Buffered Saline directly from the bottle?

Generally, it's advisable to transfer an appropriate amount of DPBS into a sterile container when using it for cell cultures or experimental procedures. This practice minimizes the risk of contamination as touching or repeatedly opening the original bottle can introduce microorganisms. Additionally, having a portion in a separate sterile container allows for more controlled and accurate volumes when performing various experimental tasks.

What are the safety considerations when using DPBS?

Dulbecco's Phosphate Buffered Saline is considered safe for laboratory use; however, standard laboratory safety practices should always be followed. It's important to use personal protective equipment, such as gloves and goggles, when handling the solution. Additionally, always dispose of any unused or expired DPBS according to your local regulations for biological waste, ensuring a safe laboratory environment for all personnel.

Can DPBS be used for in vitro experiments?

Yes, Dulbecco's Phosphate Buffered Saline is widely utilized in in vitro experiments due to its ability to closely mimic physiological conditions. It provides a stable environment without interfering chemicals, making it ideal for assays, cell culture, and various other biological experiments. Whether you are culturing eukaryotic or prokaryotic cells, using DPBS helps maintain consistency and reliability in experimental results.

Where can I buy Dulbecco's Phosphate Buffered Saline 1X DPBS in Montenegro?

You can buy Dulbecco's Phosphate Buffered Saline 1X DPBS, Without Calcium and Magnesium, pH 7.4, 500 mL, Lab Grade at Ubuy in Montenegro. Ubuy offers a convenient shopping experience with a variety of lab supplies, and you can be assured of quality products to meet your laboratory needs.

How to Shop Dulbecco's Phosphate Buffered Saline (1X DPBS), Online From Ubuy?

It’s easy to shop Dulbecco's Phosphate Buffered Saline (1X DPBS), online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Dulbecco's Phosphate Buffered Saline (1X DPBS), Available to Shop Online in Montenegro?

Yes, at Ubuy Montenegro this product is available for you to shop at a reasonable price. The Dulbecco's Phosphate Buffered Saline (1X DPBS), is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

Albert Bio

Dulbecco's Phosphate Buffered Saline (1X DPBS), Without Calcium and Magnesium, pH 7.4, 500 mL, Lab Grade

Ready-to-use DPBS solution that does not contain calcium or magnesium, suitable for general use in the laboratory.

Albert Bio

Item #: 84926563

Dulbecco's Phosphate Buffered Saline (1X DPBS), Without Calcium and Magnesium, pH 7.4, 500 mL, Lab Grade

86% of respondents would recommend this to a friend

Item #: 84926563

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What Stands Out

Ideal Buffering

Formulated for optimal pH 7.4 stability, ensuring reliable performance in cell culture and laboratory experiments, promoting reproducible results across various biological applications.

Calcium/Magnesium Free

Excludes calcium and magnesium, preventing unintended cellular responses, making it perfect for experiments requiring precise control over ionic conditions, enhancing protocol accuracy.

Convenient Volume

Comes in a 500 mL bottle, providing ample solution for extensive lab use while ensuring fresh, high-quality reagent specifically designed for continuous lab-grade applications.

Product Details

Package Weight

2.53 Pound

Who Should Buy?

Cell Culture
Ideal for maintaining and washing mammalian cells in culture due to its isotonic and buffered nature.
Biological Experiments
Suitable for use in various biological experiments requiring a stable pH and saline environment without calcium or magnesium.
Laboratory Research
A dependable reagent for researchers needing a standard buffer solution for their laboratory needs in various assays.

In Vivo Use
Not suitable for in vivo applications as it lacks nutrients and necessary components for living organisms.

Product Description

Dulbecco's Phosphate Buffered Saline (1X DPBS), Without Calcium and Magnesium, pH 7.4, 500 mL, Lab Grade

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Customer Questions & Answers

Question: How to Shop Dulbecco's Phosphate Buffered Saline (1X DPBS), Online From Ubuy?

Answer: It’s easy to shop Dulbecco's Phosphate Buffered Saline (1X DPBS), online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Dulbecco's Phosphate Buffered Saline (1X DPBS), Available to Shop Online in Montenegro?

Answer: Yes, at Ubuy Montenegro this product is available for you to shop at a reasonable price. The Dulbecco's Phosphate Buffered Saline (1X DPBS), is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

Albert Bio Buffers Editorial Review

Dulbecco's Phosphate Buffered Saline (1X DPBS), Without Calcium and Magnesium, pH 7.4, 500 mL, is an essential lab-grade solution provided by Albert Bio, designed to maintain cell viability and physiological conditions for biological experiments. The solution comes in a convenient 500 ml bottle, making it easy to measure and use for various applications such as cell culture and molecular biology. Users appreciate its reliable quality and optimal pH balance for various laboratory needs, ensuring it supports a range of experiments efficiently. This product is presented in a sturdy bottle, enhancing usability and storage in lab environments.

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Pros

Lab grade quality for reliable results
Optimal pH for physiological conditions
Convenient 500 ml bottle size
Suitable for cell culture applications
Easy to use for molecular biology experiments

Cons

Limited to specific applications in research labs

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

Ready-to-use DPBS solution
Does not contain calcium or magnesium
Easy to handle
Suitable for general use in the laboratory (not sterile)
Made in USA
Concentration: 137 mM NaCl, 10 mM Na2HPO4, 2.7 mM KCl, 1.8 mM KH2PO4

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Dulbecco's Phosphate Buffered Saline (1X DPBS), Without Calcium and Magnesium, pH 7.4, 500 mL, Lab Grade

€ 27

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Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

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What Stands Out

Product Details

Who Should Buy?

Product Description

Customer Questions & Answers

Albert Bio Buffers Editorial Review

Customer Reviews & Ratings

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Pros

Cons

Product Price History

Important information

€ 27

Features & Benefits

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